A Biuret Method for Determining Fish Muscle Proteins
نویسندگان
چکیده
منابع مشابه
The measurement of total serum proteins by the Biuret method.
The biuret reaction for proteins provides a simple and precise method for measuring serum proteins; Beer's law is obeyed to at least 10 g per dl. Several stable biuret reagents are available. Hemoglobin is the only important cause of interference which cannot be minimized by use of a sample blank. The mechanism of the biuret reaction is described and attention is drawn to the heterogeneity of t...
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In 1932 (1) the author published a method for determining methionine in proteins based on the recovery of methyl iodide. Alcoholic silver nitrate was used as absorbant and the excess of silver nitrate was titrated with thiocyanate after filtering off the silver iodide. This method was quite satisfactory and has been used recently by Barritt (2) for the determination of methionine in wool. Certa...
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A biuret method has been developed which provides quantitative measurements of protein in normal urine without interference from drugs or pigments. This method is intended for use in monitoring clinical trials of new drugs-to detect nephrotoxicity. Protein is precipitated from duplicate samples of urine by addition of cold ethanolic phosphotungstic acid. The protein precipitates are separated b...
متن کاملDextran interference in biuret-based assays for serum proteins.
We found the Easy very easy to run and without te#{233}hnique-dependent steps in the procedure. Operators need not have a high level of skill. Calibration is stable for one month, except for the ion-selective electrodes, which must be calibrated every two days. In conclusion, we found the Easy satisfactory in operational and analytical performance. The analyzer is well suited for use in the eme...
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We evaluated a rate colorimetric method (Beckman) for measuring total protein in cerebrospinal fluid. The automated instrument we used was Beckman's ASTRA TM. A 100-microL sample of spinal fluid is introduced into the biuret reagent in the reaction cell and the increase in absorbance at 545 nm is monitored for 20.5 s. Solid-state circuits determine the rate of alkaline biuret-protein chelate fo...
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ژورنال
عنوان ژورنال: NIPPON SUISAN GAKKAISHI
سال: 1955
ISSN: 1349-998X,0021-5392
DOI: 10.2331/suisan.21.284